Forwards Zoe Aldcroft (Gloucester-Hartpury, 32 caps), Sarah Bern (Bristol Bears, 46 caps), Hannah Botterman (Saracens, 30 caps), Shaunagh Brown (Harlequins, 27 caps), Poppy Cleall (Saracens, 57 caps) Amy Cokayne (Harlequins, 64 caps), Vickii Cornborough (Harlequins, 70 caps), Lark Davies (Bristol Bears, 41 caps), Rosie Galligan (Harlequins, 7 caps), Sarah Hunter (Loughborough Lightning, captain, 135 caps), Sadia Kabeya (Loughborough Lightning, 4 caps), Laura Keates (University of Worcester Warriors, 62 caps), Alex Matthews (Gloucester-Hartpury, 51 caps), Maud Muir (Gloucester-Hartpury, 11 caps), Cath O’Donnell (Loughborough Lightning, 20 caps), Marlie Packer (Saracens, 84 caps), Connie Powell (Gloucester-Hartpury, 5 caps), Morwenna Talling (Loughborough Lightning, 4 caps), Abbie Ward (Bristol Bears, 56 caps).
Backs Holly Aitchison (Saracens, 10 caps), Jess Breach (Saracens, 23 caps), Abby Dow (Wasps, 24 caps), Zoe Harrison (Saracens, 40 caps), Tatyana Heard (Gloucester-Hartpury, 5 caps), Leanne Infante (Saracens, 52 caps) Ellie Kildunne (Harlequins, 25 caps), Claudia MacDonald (Exeter Chiefs, 20 caps), Sarah McKenna (Saracens, 42 caps), Lucy Packer (Harlequins, 5 caps), Helena Rowland (Loughborough Lightning, 5 caps), Emily Scarratt (Loughborough Lightning, vice-captain, 103 caps), Lydia Thompson (University of Worcester Warriors, 54 caps)